Overview of EM radiation
EM radiation & UV
spectroscopy-introduction
electronic transition
Analysis of molecular data-spectrophotometric techniques-UV
Energy, wavelength and frequency of EM and applications of UV and rotational/vibrational states
Dual beam UV & molar extinctions & spectral variations comes from closely related small organic compounds
Red and blue shift-UV spectral shift; electronic transitions with examples
peptide bond formation & protein chromophores
chromophores:carotenoids, porphyrins in proteins and genetic materials
Factors affecting UV absorption/red and blue shift
Calculation of transmittance & Determination of absorbance & fluorescence spectroscopy
IC and ISC transitions and fluorophores
Fluorescence spectroscopy: 90 and 45 degree geometry
intrinsic and extrinsic fluorophores/FRET
BRET & CD spectroscopy/ORD
CD spectra analysis
IR spectroscopy
Analysis of IR spectra/amide vibrations
Analysis of IR spectra/theoretical simualtions
Raman spectroscopy
spectroscopic techniques: structure and interaction
ITC data analysis
X-ray crystallography: molecular structure analysis
Bragg's law /diffraction pattern/nature of crystals
crystallization
factors that affect crystallization
Unit cells/asymmetric units
symmetries in crystal & phase problem in X-ray crystallography
symmetry equivalents and space groups
space group-orthorhombic system/Mathews coefficients
structure factor and electron density calculation
ESI-MS problems
problems revision
mass spec-problems
Pre-recorded videos (folders)
